
National University of Singapore
Food Science & Technology
Titratable acidity of yoghurt
Material
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Yoghurt
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Phenolphthalein
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0.1 M standardised sodium hydroxide
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Weighing balance
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White porcelain basin
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Magnetic stirrer
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10 ml measuring cylinder
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25 ml Burette
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Plastic dropper
Procedures:
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Accurately weigh 10.0 ± 0.1 g sample of the yoghurt into two white porcelain basin. Add 10 ml of water and stir thoroughly with magnetic stirrer to ensure complete mixing.
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Add 1.0 ml of the phenolphthalein indicator into each of the porcelain basin and mix thoroughly by stirring with a magnetic stirrer.
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Rapidly, and with constant stirring, add 0.1 M NaOH from a burette until a faint pink colour remains for at least 10 s. Note the volume of alkalis used and repeat the titration. The time for the titration should not exceed 30 s for each sample and the results should agree within 0.05 ml.
ATTENTION:
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Ensure that the pink colour remains for at least 10 s.
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Ensure thorough mixing of the yoghurt and NaOH during titration.
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Ensure drop by drop addition of NaOH to prevent overshoot of titration.
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Ensure that the burette is not slanted during titration to ensure proper reading of the volume of the NaOH used.
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Ensure sufficient time is allocated between each addition of NaOH as the yoghurt can be quite viscous.