
National University of Singapore
Food Science & Technology
For the determination of the stability of chlorophyll:
Materials:
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Frozen peas
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Canned peas
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Vinegar
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1 M NaOH
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NaHCO3
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Weighing boat
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250 ml Beaker
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Heating Plate
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Spoon
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Stopwatch
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10 ml Measuring Cylinder
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pH meter
Procedure:
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Heat 150 ml of deionised (DI) water to boiling.
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Add approximately 25 g frozen peas.
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Bring the water to boil, continue heating for 7 minutes.
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Remove the sample from the water and place in the weighing boat.
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Add 10 ml of vinegar to about 150 ml DI water and determine the pH of the solution. Boil the solution and repeat steps 2 to 4.
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Add 10 ml 1 M NaOH to 150 ml DI water and determine the pH of the solution. Boil the solution and repeat steps 2 to 4.
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Expose a fourth 25 g sample of peas to a cold mixture of 10 ml vinegar and 150 ml DI water for 7 minutes without cooking. Remove the sample and place in the weighing boat.
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Expose a fifth 25 g sample of peas to a cold mixture of 2 g NaHCO3 and dissolve in 150 ml DI water without cooking. Remove the sample and place in the weighing boat.
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Set up a sixth weighing boat with canned peas.
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Compare all the samples for colour and texture.
Common Mistakes Made:
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Students tend to start the timer for 7 minutes even before the water has started boiling.
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Students tend to record the pH of the solution before the pH stabilises. Ensure that the pH stabilises before proceeding to the next step.
![]() Comparison of colour changes between peas without any treatment (control, left) and peas treated with hot Sodium hydroxide (right) | ![]() Comparison of colour changes between peas without any treatment (control, left) and peas treated with hot water (right) | ![]() Comparison of colour changes between peas without any treatment (control, left) and peas treated with cold vinegar (right) |
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![]() Comparison of colour changes between peas without any treatment (control, left) and peas treated with cold Sodium bicarbonate (right) | ![]() Comparison of colour changes between peas without any treatment (control, left) and canned peas (right) | ![]() Comparison of colour changes between peas without any treatment (control, left) and peas treated with hot vinegar (right) |
![]() Overall comparison of all the peas and various treatments Top: Control. Middle (Left to right): Hot water, Hot vinegar and cold sodium hydroxide. Bottom (Left to right): Cold vinegar, cold sodium bicarbonate and canned peas. |
(Top) Video demonstrating the determination of the stability of chlorophyll