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For the determination of the stability of chlorophyll:

Materials:

 

  • Frozen peas

  • Canned peas

  • Vinegar

  • 1 M NaOH

  • NaHCO3

  • Weighing boat

  • 250 ml Beaker

  • Heating Plate

  • Spoon

  • Stopwatch

  • 10 ml Measuring Cylinder

  • pH meter

Procedure:

 

  • Heat 150 ml of deionised (DI) water to boiling.

 

  • Add approximately 25 g frozen peas.

 

  • Bring the water to boil, continue heating for 7 minutes.

 

  • Remove the sample from the water and place in the weighing boat.

  • Add 10 ml of vinegar to about 150 ml DI water and determine the pH of the solution. Boil the solution and repeat steps 2 to 4.

 

  • Add 10 ml 1 M NaOH to 150 ml DI water and determine the pH of the solution. Boil the solution and repeat steps 2 to 4.

 

  • Expose a fourth 25 g sample of peas to a cold mixture of 10 ml vinegar and 150 ml DI water for 7 minutes without cooking. Remove the sample and place in the weighing boat.

 

  • Expose a fifth 25 g sample of peas to a cold mixture of 2 g NaHCO3 and dissolve in 150 ml DI water without cooking. Remove the sample and place in the weighing boat.

 

  • Set up a sixth weighing boat with canned peas.

 

  • Compare all the samples for colour and texture.

 

Common Mistakes Made:

  • Students tend to start the timer for 7 minutes even before the water has started boiling.

 

  • Students tend to record the pH of the solution before the pH stabilises. Ensure that the pH stabilises before proceeding to the next step. 

Comparison of colour changes between peas without any treatment (control, left) and peas treated with hot Sodium hydroxide (right)

Comparison of colour changes between peas without any treatment (control, left) and peas treated with hot water (right)

Comparison of colour changes between peas without any treatment (control, left) and peas treated with cold vinegar (right)

Comparison of colour changes between peas without any treatment (control, left) and peas treated with cold Sodium bicarbonate (right)

Comparison of colour changes between peas without any treatment (control, left) and canned peas (right)

Comparison of colour changes between peas without any treatment (control, left) and peas treated with hot vinegar (right)

Overall comparison of all the peas and various treatments Top: Control. Middle (Left to right): Hot water, Hot vinegar and cold sodium hydroxide. Bottom (Left to right): Cold vinegar, cold sodium bicarbonate and canned peas.

(Top) Video demonstrating the determination of the stability of chlorophyll

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