
National University of Singapore
Food Science & Technology
Titratable acidity of milk
Material:
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Phenolphthalein
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Magnetic stirrer
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White porcelain basin
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10 ml pipette
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Plastic dropper
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25 ml burette
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0.1 M NaOH
For more information of the types of milk used, please click on the individual links.
Procedure:
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Pipette 10 ± 0.1 ml of the supplied milk (choose at least two from three samples provided: sterilised milk, UHT milk and pasteurised milk) into two white porcelain basins. This will help observe the colour change.
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Add 1.0 ml of the phenolphthalein indicator into each of the porcelain basin and mix thoroughly by stirring with magnetic stirrer.
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Rapidly, and with constant stirring, add the 0.1 M sodium hydroxide from a burette until a faint pink colour remains for at least 10 s. Note the volume of alkalis used and repeat the titration. The time for the titration should not exceed 30 s for each sample and the results should agree within 0.05 ml.
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Repeat the titration and calculations with the second milk sample.
(Top) Demonstration video on determining the Titratable acidiy of milk