National University of Singapore
Food Science & Technology
Membrane separation - Experimental setup
Materials:
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Sartorius Sartoflow Alpha Filtration system
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Hydrosart® Ultrafiltration 10 kD (NMWCO)
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Refractometer
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UV-Vis spectrophotometer
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Measuring cylinders
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Stopwatch
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Mango juice concentrate or any other juice
(Top) Demonstration video for performing the experiment
Determine the permeability coefficient of the membrane
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Fill feed container with 3 L DI water
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Switch on the pump to achieve desired inlet pressure and retentate pressure
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Take note of the volume of the feed, retentate and permeate. Determine the permeate flow rate for the volume of permeate collected with the time period
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Repeat with varying retentate pressure to achieve more data points
Separation efficiency of ultrafiltrations system
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Prepare 5 L of 5%(w/w) and 10%(w/w) maltodextrin solution
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Fill the feed and adjust feed pressure to desired pressure
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Adjust retentate pressure to desired pressure
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Measure the volume change of the feed, retentate and permeate under various pressure levels within a time period
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Measure the solute concentration of the permeate and the retentate, respectively.
Separation of fruit juice
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Prepare 2 L of grape juice as feed. Collect about 10 ml for UV-VIS measurement
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Adjust retentate and feed pump pressure to desired
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Measure the volume of the feed, retentate and permeate.
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Measure the absorbance of the feed, retentate and permeate using a UV-VIS spectrophotometer